HANNAH OLUWAKEMI OLADIPO

Analytical Research Scientist with many years of educational and hands-on experience in experimental research and data analysis. Detail-oriented and methodical professional successful in developing in-depth research projects. Hardworking professional with extensive training in cancer research, nucelear-cytoplasmic transport, RNA-protein interaction, genomics and protein-protein interaction. Reliable team player with excellent communication and problem-solving skills.

Doctoral fellow

Rhodes University, Grahamstown, South Africa : 2018-2022

Research tittle: The role of HOP/STIP1 in nuclear-cytoplasmic transport

Supervisor: Professor Adrienne Edkins

• Human cell line tissue culture, including HEK293T, HeLa, and HCT116
• Designed and cloned fluorescently tagged nucleoporins expression plasmids
• mRNA Fluorescence in-situ hybridization (FISH) analysis
• Fluorescence and confocal microscopy to study nuclear pore integrity, nuclear-cytoplasmic distribution of nucleoporins, NES/NLS tagged proteins and nuclear export factors
• Luciferase activity of constitutive transport element (CTE)-containing transcripts
• Immunoprecipitation analysis of nucleoporins and nuclear export factors in wild type and Hop depleted cells
• Drug treatment and cytotoxicity analysis
• Quantitative polymerase chain reaction (qPCR) and polymerase chain reaction (PCR)
• SDS PAGE and Western blot analysis
• Bioinformatics analysis: expression and survival analysis of Hsp70, Hsp90 isoforms, and Hop in TCGA cancer
• Protein-protein interaction and identification of hub genes co-expressed with Hsp70, Hsp90 isoforms, and Hop in TCGA cancer
• Identification of dysregulated proteins in Hop depleted and overexpressing cells
• Identification of Hsp70 and Hop interactome
• Undergraduate student tutoring

Postgraduate studies

Saarland University, Saarland Germany: 2014-2016

Identification of important physicochemical properties of antibiotics and antineoplastic drugs based on their gene ontology categories: a machine learning approach

Supervisor: Professor Dr. Volkhard Helms

• Data retrieval from DrugBank database
• Gene ontology analysis
• Principal component analysis
• K-means clustering analysis
• Machine learning: Decision tree analysis

Research Assistant

Chair of computational biology, University of Saarland: July-December,2016

Advisor Name: Professor Dr. Volkhard Helms

Project: Antineoplastics and Antibiotics drugs analysis in cancer

• Data search and bioinformatics analysis

Student internship August-September, 2015

Dept. of Applied Bioinformatics, German Cancer Research Centre (DFKZ), Heidelberg, Germany

Advisor: Prof E. Adebiyi, Prof Benedikt Brors

Project: Assessment of plasmodium falciparum RNA pseudoridylate synthase (putative) as novel drug target

· Primer Design for PCR

· Cloning of Pseudouridylate synthase into peT28a plasmid vector

· Sequencing of cloned plasmid

Merck innovation center, Merck KGaA, Darmstadt, Germany June-December 2017

· Data gathering and resource person for the accelerator teams

Journal Publications

• Seyi Ebun Adeboye, Shakirat O. A Ajenifujah-Solebo, Hannah Oluwakemi Oladipo, Dolapo Adetokunbo Adeshina, P. (2022).Case study on Risk and Benefit of Genetically Modified Nitrogen-use Efficient Water-use Efficient and Salt-tolerant Rice Breed in Nigeria. International Journal of Environment Agriculture and Biotechnology (ISSN: 2456-1878).7(3), 139-146.10.22161/ijeab.73.19
• Rowaiye, A., Okpalefe, O., Onuh, O., Ogidigo, J., Oladipo, O., Ogu, A., ... & Onyekwere, O. (2020). Preparing for the Storm: Mitigating the Effect of SARS-CoV-2 Induced Hypercytokinemia. https://www.preprints.org/manuscript/202011.0604/v1
• Oladipo, O. H., Ibrahim, R. R., Adeboye, S. E., & Kuiper, H. (2020). Readiness of the Nigerian public for the introduction of genetically modified crops into the food market. African Journal of Biotechnology, 19(7), 426-438.
• Ibrahim, R. R., & OLADIPO, O. H. (2020). Forecasting the spread of COVID-19 in Nigeria using Box-Jenkins Modeling Procedure. medRxiv.
• Igwe, J, Onaolapo, J. , Kachallah, M., Nworie, A. , Oladipo, H. , Ojiego, B. , Enose, O. , Adeboye, S. , Durowaiye, M. , Akpa, A. and Ibanga, I. (2014) Molecular Characterization of Extended Spectrum β-Lactamase Genes in Clinical E. coli Isolates. Journal of Biomedical Science and Engineering, 7, 276-285. doi: 10.4236/jbise.2014.75030.
• Igwe, J.C., Onaolapo, J.A., Dauda, E.O. and Oladipo, H.O. (2013) Plasmid Conjugation in E. coli and Drug Resistance Plasmid Conjugation in E. Coli and Drug Resistance. Nig J. Biotech. Vol. 26 (2013) 41- 49 ISSN: 0189 17131
• Conference and seminar presentations
• 14th International Symposium on the Biosafety of Genetically Modified Organisms (ISBGMO14) Guadalajara 2017 "Readiness for the introduction of genetically modified crops into the food market."
• Phytochemical and antibacterial screening of selected medicinal plants used in the treatment of sexually transmitted diseases in rural areas of Abuja and Sokoto town: Presented at the 26th annual National Conference of Biotechnology Society of Nigeria, (NABDA BSN 2013)
• Antibacterial screening of maggot excretions/secretions of Lucilia sericata against selected bacteria: Presented at the 26th annual National Conference of Biotechnology Society of Nigeria (NABDA BSN 2013)
• Poster presentation: Erythropoietin production in tobacco plants; genetically modifying tobacco to meet market demands (Masters seminar)
• Seminar Presentation: Medicinal uses of alkaloids (Bachelors seminar)

Workshop
•"Cancer Bioinformatics Tools," 9th December 2022 ICGEB course, Muğla Sıtkı Koçman University, Turkey
• Advanced Proteomics Workshop, 20th August 2018 – 22nd August 2018, at the University of the Witwatersrand (South Africa), by Prof Kathryn Lilley (University of Cambridge, UK), Prof Stephen Pennington (University College Dublin, Ireland), and Prof David Tabb (Stellenbosch)
• H3ABioNet Postgraduate Bioinformatics Workshop 21st April-26th May 2014, Covenant University Bioinformatics Research (CUBRe), Ota Nigeria
• "Applications of bioinformatics in modern molecular biology," 21st-25th October 2013: Organized by the International Centre For Genetic Engineering and Biotechnology(ICGEB), New Delhi, India

Antibacterial activity of excretions/secretions of Lucilia sericata against selected bacteria

Supervised by: Dr Helen Hopper, Northumbria University

Lucilia sericata is used for therapeutic purposes in the treatment of chronically infected wounds. The larva of this fly heals wounds via wound debridement, disinfection and stimulation of wound healing by excretion of antibacterial and growth-promoting substances. The project was aimed at studying the antibacterial properties of the excretions/secretions (ES) of L.sericata against Methicillin resistant staphylococcus aureus (MRSA), Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. Three bioassays were used: zone of inhibition, colony-forming unit, and turbidometric assay. ES was obtained using larvae that has been exposed to bacteria infested porcine tissue and also from larvae that had never been in contact with bacteria.

Determination of 3D-structure of C.japonicus Pectate Lyase10A by X-ray Crystallography

Supervised by: Prof Gary Black, Northumbria University

Cellvibrio japonicus is a good source of highly efficient Pel10A which are widely applied in industrial processes. E.coli is also an efficient expression host for production of large quantity of recombinant Pel10A. Application of X-ray crystallography in studying the 3D structure of the catalytic module of Pel10A provides more understanding of its catalytic features. This technique requires large amount of high-quality crystals of pel10A, and the experiment was aimed at expression, purification, characterization, and crystallization of the catalytic module of pel10A. E.coli was transformed with pET-28a plasmid containing Pectate lyase (Pel10A) gene. Biochemical assay, electrophoresis, and crystallization analysis were carried out on the expressed recombinant protein.

• Ph.D. scholar: DAAD In-Country/In-Region Scholarship Programme, Rhodes University, South Africa: Full scholarship for doctoral studies 2018-2022
• Student Travel grant: 14th International Symposium on the Biosafety of Genetically Modified Organisms (ISBGMO14) Guadalajara 2017

· Mentoring: Nine-Tenths Matric Mentoring Programme, Grahamstown, South Africa

· Newcastle city ambassador supporter for the London 2012 Olympics in Newcastle, United Kingdom

• Cooking
• Travelling
• Volunteering

• Prof Adrienne Edkins
  Biomedical Biotechnology Research Unit (BioBRU)
  Rhodes University,
  Grahamstown, Eastern Cape, South Africa.
  Telephone: +27-46-603-8446
  Fax: +27-46-603-7576
  Email: a.edkins@ru.ac.za
  • Prof Dr Volkard Helms
  Chair computational biology, Centre for Bioinformatics, University of Saarland
  Telephone: +49 681 302 70701, Fax: +49 681 302 70702
  Center for Bioinformatics
  Campus E2 1, R. 315, P.O. Box 15 11 50
  D-66041 Saarbrücken, Germany
  Email: volkhard.helms@bioinformatik.uni-saarland.de
  • Dr Rinke Vinkenoog
  Address: School of Life Sciences, Northumbria University,Ellison Building
  Ellison Place,Newcastle upon Tyne, NE1 8ST
  Tel: +44 (0) 191 227 3508, Fax: +44 (0) 191 227 3519
  Email: rinke.vinkenoog@northumbria.ac.uk
  • Dr Adekunle Rowaiye
  Fellow, College of Veterinary Surgeons of Nigeria (FCVSN)
  Fellow, Agriculture Graduates Association of Nigeria (FAGAN)
  Deputy Director, Dept. of Medical Biotechnology
  National Biotechnology Development Agency (NABDA)
  Federal Ministry of Science and Technology (FMST)
  Airport Road, Lugbe
  Abuja - Nigeria
  Phone: +2348023287179
  Email: adekunlerowaiye@gmail.com