GIDEON OLORUNWUNMI MORONKEJI

• I oversee the laboratory to ensure that it is well organized for proper functioning
• I appropriately collect haematology, bacteria, fungi, and viral sample for proper diagnostic
• I conduct the microbial culture, identification and sample isolation
• I collate the results of patients to examine the geographical disease and infection spread for medical report
• I train the intern students on molecular biology review and sample handling in the laboratory
• Improves diagnostic accuracy with meticulous sample analysis.
• Complies with health and safety regulations, reducing risk of accidents or contamination.
• Keeps up-to-date with latest scientific developments through constant learning and development opportunities.
• Documents test results in laboratory logs and databases with strict adherence to data security protocols.
• Work extended hours during emergencies ensuring continuous healthcare service provision.

• Retrieving lung cancer sequence from NCBI Database, using the chromosome 7 GenFull Bank for variant calling and analysis of codon change and mutation
• Hands-on: Sequence retrieval and RefSeq from NCBI database
• Call complementary strands
• Protein Translation
• Mapping Reference
• Genome annotation
• Variant calling analysis
• ORF analysis
• Primer and Probe design
• Tableau and ClustVis for Heat map analysis
• Demonstrated adaptability by successfully balancing part-time work commitments with academic responsibilities.
• Conducted research for final year project, developed analytical skills.

• Ibadan Summer Studies in Genomics & Bioinformatics, Aix-Marseille Uni, France
• Hands-on: Bacteria DNA extraction, DNA Quality control, Illumina sequencing, Galaxy for genome sequence analysis, ORF for codon reading, Nucleotide BLAST on NCBI, NCBI virus accession number downloads

Molecular detection of Human Boca Virus (HBoV) in out-patients’ children with underlying symptoms.

• Followed up with daily activities on sample collection and reagent procurement.
• Nasopharyngeal swab sample collection from 250 out-patient children.
• Extracted DNA genome from 250 nasopharyngeal swab samples.
• Aliquoted the One-Step Master mixture for thermocycling.
• Initiated one-step master mixture (RT-PCR)on Rotor-Gene Q thermocycler machine.
• Detected positive genome via Cycle Threshold value.

• Molecular Detection of Human metapneumovirus (hMPV) in HIV patients
• Nasopharyngeal swab sample were collected from 150 patients
• Extracted RNA genome from 150 nasopharyngeal swab samples
• Aliquoted the One-Step Master mixture for thermocycling
• Initiated one-step master mixture (RT-PCR) on Rotor-Gene Q thermocycle machine
• Detected positive genome via Cycle Threshold value

• Molecular detection of Influenza D virus in cattle in Lagos, Nigeria
• Nasopharyngeal swab sample and data of influenza-like symptoms were collected from 80 cattle
• Transported to lab., aliquoted in VTM, and prepared for extraction
• Extracted RNA genome from 80 nasopharyngeal swab samples
• Aliquoted the cDNA and master mixture on the extracts
• Initiated the aliquot on Rotor-Gene Q thermocycler
• Target genome was detected via Gel Electrophoresis on image illuminator

• Epidemiological surveillance of Adenovirus seroprevalence in HIV and tuberculosis patients
• Blood sample collection from 400 patients
• Screened for HIV and Tuberculosis infection
• Detected Adenovirus via colour change on viral serology screening plate/well